Ultramicrospectrophotometer

Hoʻolauna Huahana

ʻO ka Ultramicrospectrophotometer kahi ʻano ʻike wikiwiki a pololei hoʻi o ka waikawa nucleic, ka protein a me ka nui o ka hopena cell me ka ʻole o ka hoʻomehana mua ʻana, ʻo ka nui o ka laʻana he 0.5 a 2ul wale nō, a hiki i ke ʻano cuvette ke ʻike i ka nui o ka bacteria a me nā mea moʻomeheu ʻē aʻe. Hiki ke hoʻopili ʻia ka hana ʻike fluorescence me ka pahu loiloi quantitative fluorescence, ma o ka hui pū ʻana o nā dyes fluorescent a me nā mea i manaʻo ʻia hiki ke helu pololei i ka DNA, RNA a me nā nui o ka protein, a hiki i ka liʻiliʻi loa ke hiki i ka 0.5pg/μl (dsDNA).

Nā hiʻohiʻona huahana

He pōkole ke alapine (frequency) o ke kukui uila, a hoʻohālikelike ʻia me ke ʻano ʻike kuʻuna e hoʻonui i ke ola lawelawe o ke kumu kukui. Hiki i ka hoʻoulu ʻana i ka ikaika o ka mālamalama o nā huahana hoʻāʻo liʻiliʻi ke ʻike wikiwiki, ʻaʻole maʻalahi e hōʻino.;

Hana Fluorescence: Me ka fluorescence quantitative reagent hiki ke ʻike i ka pg concentration dsDNA;

4 ʻenehana ʻike ala optical: ʻenehana mana kaʻa kū hoʻokahi, hoʻohana i ke ʻano ʻike ala optical "4", paʻa, hana hou, ʻoi aku ka maikaʻi o ka linearity, ʻoi aku ka nui o ka laulā ana;

Paʻi i kūkulu ʻia: Me nā koho ʻikepili-i-ka-paʻi maʻalahi e hoʻohana, hiki iā ʻoe ke paʻi pololei i nā hōʻike mai ka paʻi i kūkulu ʻiar;

ʻO ka hopena bacteria OD600, ka ʻike ʻana i nā microbial: me ka ʻōnaehana ʻike ala optical OD600, ma mua o ke ʻano kīʻaha he kūpono no ka bacteria, nā microorganism a me nā ʻike ʻana i ka hoʻohuihui hopena moʻomeheu ʻē aʻe;

Ka hana hou ʻana a me ka linearity kiʻekiʻe;