Mea Paʻa Pahu Hoʻāʻo Hoʻokahi No ka Hoʻokaʻawale ʻAkika Nucleic

Hoʻolauna Huahana

Hāʻawi ka pahu hoʻokaʻawale waikawa nucleic MagPure i kahi ala maʻalahi loa, wikiwiki a kūpono hoʻi no ka hoʻokaʻawale ʻana o ka DNA a i ʻole RNA ma ke ʻano kiʻekiʻe ma muli o ke ʻano o nā beads magnetic. ʻAʻole loaʻa i ka pahu hoʻokaʻawale waikawa nucleic MagPure kahi mea hoʻoheheʻe organik ʻino a kūpono loa ia no ka hana ʻana i nā ʻano laʻana like ʻole. Hoʻopau kēia ʻenehana ponoʻī i ka pono no ka centrifugation, ka kānana vacuum a i ʻole ka hoʻokaʻawale kolamu, no laila e hoʻonui ai i ka throughput laʻana a hoʻomaikaʻi i ka hana hou ʻana. Ua mākaukau ka DNA a i ʻole RNA i hoʻomaʻemaʻe ʻia e MagPure no nā ʻano noi biology molecular āpau e like me PCR, sequencing, blotting procedures, mutant analysis a me SNP. He kūpono ka pahu hoʻokaʻawale waikawa nucleic MagPure no ka hoʻohana ʻana me ke koko i mālama pinepine ʻia me nā anticoagulants e like me citrate, heparin a i ʻole EDTA, nā wai olaola, nā ʻiʻo Paraffin-enbedded, nā ʻiʻo holoholona a mea kanu paha, nā cell i hoʻoulu ʻia, nā cell bacteria e lawe ana i ka plasmid a me ka laʻana virus. Hoʻohana ʻia ka pahu hoʻokaʻawale waikawa nucleic MagPure me hoʻokahi protocol maʻamau-hoʻomākaukau laʻana, ka hoʻopaʻa magnetic, ka holoi a me ka elution. A ma ke kākoʻo ʻana i ka hoʻohana ʻana i nā mea hana hoʻomaʻemaʻe BigFish NUETRACTION, hoʻokō nā mea kūʻai aku i ka unuhi DNA a i ʻole RNA wikiwiki a kiʻekiʻe.