ʻO ka pahu hoʻomaʻemaʻe DNA ʻo MagPure Bacteria Genomic

Wehewehe Pōkole:

Ma hope o ka mālama ʻia ʻana me ka wai hoʻoheheʻe, hoʻokuʻu ʻia ka waikawa nucleic i loko o ka hāpana bacteria me ka hoʻohana ʻana iā Lysis Buffer. Hoʻopaʻa wale ʻia ka DNA genomic i hoʻokuʻu ʻia a pili pono i nā momi Magnetic. Hoʻopaʻa ʻia ka DNA genomic i hoʻopaʻa ʻia i nā ʻāpana magnetic e nā mea magnetic; wehe ʻia nā mea haumia ma ka holoi ʻana me Wash Buffer. A laila e hoʻokuʻu ʻia ka waikawa nucleic mai nā ʻāpana me kahi Elution Buffer. Pili no nā ʻano bacteria gram-negative a me nā bacteria maikaʻi, a pēlā aku.

KĀHEA IĀ MĀ˚OU

Nā kikoʻī huahana

Nā Lepili Huahana

ʻĀpana nui

Pōpoki.#	BFMP10R1	BFMP10R16	BFMP10R	Cnā ʻāpana
Packing	1T40T/pahu	16T16T/pahu	32T32T/pahu
ʻO ka Protease K	400μ L x 2	320μ L	640μ L	Hoʻonā Protease (Hoʻopaʻa ʻokoʻa ʻia)
RNaseA	80μ L	32μ L	64μ L	Hoʻonā ʻenzyme (Hoʻopaʻa ʻokoʻa ʻia)
Buffer BA	12mL	5ml	10ml	Wai hoʻoheheʻe paʻakai
Buffer BL	I loko o nā lua he kanahā 6 hoʻomākaukau kāwele 40 mau ʻāpana.	96 luawai i hoʻopaʻa mua ʻia pā 2 ʻāpana.	96 luawai i hoʻopaʻa mua ʻia pā 2 ʻāpana.	Denaturant ikaika a me ka buffer Tris
Buffer WA				Wai hoʻoheheʻe paʻakai
WB Buffer				Wai hoʻoheheʻe paʻakai haʻahaʻa
Buffer DE				Hoʻonā Tris
Nā momi Magnetic				Hoʻonā bead magnetic hydroxy
Puke aʻo mea hoʻohana	1	1	1