Pahu Hoʻomaʻemaʻe DNA Genomic Koko MagaPure

Wehewehe Pōkole:

Loaʻa i kēia pahu nā microspheres superparamagnetic a me ka buffer extraction i hana mua ʻia, a kūpono no ka unuhi maʻalahi a me ka pono o ka genomic DNA mai nā hāpana koko piha anticoagulated hou, hau ʻia, a mālama lōʻihi ʻia. He nui nā ʻāpana DNA genomic i unuhi ʻia, maʻemaʻe loa, a he kūlana paʻa a hilinaʻi hoʻi. He kūpono ka DNA i unuhi ʻia no nā hoʻokolohua like ʻole e like me ka enzyme digestion, PCR, ke kūkulu ʻana i ka waihona puke, ka hybridization Hema, a me ka high-throughput sequencing.

KĀHEA IĀ MĀ˚OU

Nā kikoʻī huahana

Nā Lepili Huahana

Nā hiʻohiʻona o ka huahana

Ka laulā o nā noi laʻana:Hiki ke unuhi pololei ʻia ka DNA genomic mai nā laʻana e like me ke koko anticoagulated (EDTA, heparin, a me nā mea ʻē aʻe), buffy coat, a me nā clots koko.
Wikiwiki a maʻalahi hoʻi:Hana ʻia ka lysis o ka laʻana a me ka hoʻopaʻa ʻana o ka waikawa nucleic i ka manawa like. Ma hope o ka hoʻouka ʻana i ka laʻana ma luna o ka mīkini, hoʻopau ʻia ka unuhi ʻana o ka waikawa nucleic, a hiki ke loaʻa ka DNA genomic kiʻekiʻe ma mua o 20 mau minuke.
Palekana a ʻaʻohe mea ʻawahia:ʻAʻohe mea hoʻoheheʻe ʻawahia e like me ka phenol a me ka chloroform i loko o ka reagent, a he kiʻekiʻe kona palekana.

Nā mea hana hiki ke hoʻololi ʻia

ʻO Bigfish BFEX-32E/BFEX-32/BFEX-96E

Nā palena loea

Ka nui o ka laʻana:200μL
Ka hua o ka DNA:≧4μg
Ka maʻemaʻe o ka DNA:A260/280≧1.75

Nā kikoʻī

Inoa Huahana	Pōpoki. Helu.	Hoʻopili ʻana
Pahu Hoʻomaʻemaʻe DNA Genomic Blood MagaPure（Pūʻolo i hoʻopiha mua ʻia）	BFMP02R	32T
Pahu Hoʻomaʻemaʻe DNA Genomic Blood MagaPure（Pūʻolo i hoʻopiha mua ʻia）	BFMP02R1	40T
Pahu Hoʻomaʻemaʻe DNA Genomic Blood MagaPure（Pūʻolo i hoʻopiha mua ʻia）	BFMP02R96	96T