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Magire Aquatic Animal Genomic DNA
KA HOOMAHA
Ua hoʻopili kēia pahu i kahi pahu i hoʻomohalaʻia a hoʻolālāʻia i nā'ōnaehana kīwaha me nā bead i hoʻopaʻaʻia i nā binds kūikawā i dna. Hiki iā ia ke kāohi koke, Adsorb, hoʻokaʻawale, ae hoʻomaʻemaʻeʻo Plucika i nā holoholona nui. He kūpono kūpono no ka hoʻokaʻawaleʻana a me ka hoʻomaʻemaʻeʻana iā Genomic DNA mai nāʻano manu manu likeʻole, a hiki ke wehe i nā mea hoʻonaninani, a me nā mea kanu likeʻole. Ma ke kākoʻoʻana i ka hoʻohanaʻana i ka nui o nā bigfish bed nucleic acid godictor ʻO ka DNAMAICT DNAMIC DNA i loaʻa i ka maʻemaʻe kiʻekiʻe a maikaʻi hoʻi, a hiki ke hoʻohana nuiʻia i loko o ka pcrbdization scrr / Qpcr,
Nā hiʻohiʻona o ka huahana
◆ Nā koho ākea ākea: hiki ke hoʻokaʻawaleʻiaʻo Genomic DNA i nāʻano holoholona'ē aʻe
◆ E mālama pono a me ka meaʻoleʻole:ʻaʻole i loaʻa ka reagent i nā mea i hoʻopaʻaʻia e like me ka phenol a me ka chloroform
◆ Autoat: pono me ka nui o ka bigfish nuklecticat stidractor, hiki iā ia ke hana i nā kiʻi kiʻekiʻe
◆ Marity keʻokeʻo: hiki ke hoʻohana pololeiʻia no nā hoʻokolohua molicular biology e like me ka pcr, etzyme edbridition, etcbdization, etcridome
Nā kaʻina hana no ka hoʻoiliʻana
Sampling: E lawe i 25-30 mg o nā'āpana holoholona aquatic
Grinding: E kahe ana ka night
Digestion: 56 ℃ ℃ ihana mahana wela
Ma ka mīkini:ʻo ke kauʻana i ka supernatant a hoʻohui iā ia i ka pā no ka loaʻaʻole
Nā poʻoaʻenehana
Hōʻikeʻike i Sage: 25-30MG
DNA MINIty: A260 / 280 ≧ 1.75
Hoʻoponopono i nā mea hana
Bigfish BFFX-32 / BFEX-32E / BFFEX-96Y
ʻO ka kiko'ī o ka huahana
| Hua waihona | Cat. ʻAʻole. | Paliole |
| MagaMaluhiaʻO nā holoholona holoholona aquaticDna hoʻomaʻemaʻe(pUa hoʻopiha houʻia) | Bfm21R | 32T |
| MagaMaluhiaʻO nā holoholona holoholona aquaticDNA PRIETIFIT SITI (BEP-HOPE FIKIL FORET) | Bfm21R1 | 40T |
| MagaMaluhiaʻO nā holoholona holoholona aquaticDNA PRIETIFIT SITI (BEP-HOPE FIKIL FORET) | Bfm21R96 | 96T |
| Rnase a(pUrchase) | BFD017 | 1ml /tupu (10mg / ml) |
Hōʻano Pūnaewele