Ka Hoʻokaʻawale ʻana i ka DNA/RNA

Hoʻolauna huahana:

Ma ka hoʻohana ʻana i ka ʻenehana hoʻomaʻemaʻe bead magnetic, hiki i ka pahu hoʻomaʻemaʻe DNA/RNA virus Magpure ke unuhi i ka DNA/RNA o nā ʻano maʻi like ʻole e like me ka maʻi African Swine Fever a me ka coronavirus hou mai nā ʻano laʻana like ʻole e like me ka serum, plasma a me ka hopena hoʻokomo swab, a hiki ke hoʻohana ʻia ma ka PCR/RT-PCR downstream, sequencing, polymorphism analysis a me nā hoʻokolohua ʻē aʻe o ka nucleic acid a me ka ʻike ʻana. Hoʻolako ʻia me ka mea hana hoʻomaʻemaʻe nucleic acid piha piha ʻo NETRACTION a me ka pahu hoʻouka mua, hiki ke hoʻopau koke i ka unuhi ʻana o kahi helu nui o nā laʻana o ka nucleic acid.

Nā Hiʻohiʻona Huahana:

1. Palekana e hoʻohana, me ka ʻole o ka reagent ʻawahia
2. Maʻalahi e hoʻohana, ʻaʻohe pono no Proteinase K a me Carrier RNA
3. E unuhi koke a me ka maikaʻi i ka DNA/RNA viral me ke koʻikoʻi kiʻekiʻe
4. E lawe a mālama i ka mahana o ka lumi.
5. Kūpono no nā ʻano hoʻomaʻemaʻe waikawa nucleic viral like ʻole
6. Hoʻolako ʻia me ka mea hana hoʻomaʻemaʻe waikawa nucleic NUETRACTION piha piha e hana i 32 mau laʻana i loko o 30 mau minuke.