Pahu ʻIke Waikawa Nukleika SARS-CoV-2 (Fluorescence RT-PCR)

Wehewehe Pōkole:

Hiki ke hoʻohana ʻia ka pahu ʻike waikawa nucleic SARS-COV-2 (Fluorescence RT-PCR) no ka ʻike ʻana i ka waikawa nucleic o ka coronavirus hou, hoʻohana ʻia no ka ʻike kōkua a me ka noiʻi epid-emiological o ka maʻi coronavirus hou, kūpono no CDC, nā haukapila, ke keʻena hoʻokolohua lapaʻau ʻaoʻao ʻekolu, ke kikowaena hoʻokolokolo kino a me nā keʻena hoʻokolohua lapaʻau ʻē aʻe.

KĀHEA IĀ MĀ˚OU

Nā kikoʻī huahana

Nā Lepili Huahana

Nā Hiʻohiʻona Huahana

1, ʻIke kiʻekiʻe: Palena o ka ʻIke (LoD)＜2 × 102 kope/ml.

2, ʻO ka gene pahuhopu pālua: ʻIke i ka gene ORFlab a me ka gene N i hoʻokahi manawa, e hoʻokō me ke kānāwai WHO.

3, Kūpono no nā mea hana like ʻole: ABI 7500/7500FAST; Roche LightCycler480; BioRad CFX96; ʻO kā mākou BigFish-BFQP96/48 ponoʻī.

4, Wikiwiki a maʻalahi: He maʻalahi ka hoʻohana ʻana i ka reagent i hui mua ʻia, pono nā mea kūʻai aku e hoʻohui i ka enzyme a me ka template. Hoʻohālikelike maikaʻi ʻia ka pahu hoʻokaʻawale waikawa nucleic a Bigfish i kēia assay. Ma ka hoʻohana ʻana i ka mīkini hoʻokaʻawale piha piha, wikiwiki ka hana ʻana i nā laʻana he nui.

5, Palekana Bio: Hāʻawi ʻo Bigfish i ka Laʻana Preservative Liquid e hoʻopau koke i ka maʻi e hōʻoia i ka palekana o nā mea hoʻohana.