Ka unuhi ʻana i ka DNA lepo/fecal wikiwiki a maʻemaʻe hoʻi me ka Big Fish Sequence

ʻO ka lepo, ma ke ʻano he kaiapuni kaiaola like ʻole, ua waiwai nui i nā kumuwaiwai microbial, me nā ʻano microbial like ʻole e like me ka bacteria, fungi, virus, cyanobacteria, actinomycetes, protozoa a me nā nematodes. Loaʻa iā lākou kahi ākea o nā hana metabolic a me nā waiwai physiological, he kuleana koʻikoʻi ko lākou i ka pōʻaiapuni meaʻai o ka lepo a he mea nui ia no ka wehe ʻana i nā mea haumia lepo. Ma ke ʻano he hoʻokahi o nā kaiapuni like ʻole o ka biology ma ka honua, he mea koʻikoʻi kū hoʻokahi nā haʻawina biology molecular o ka lepo. Ma kēia kaʻina hana, ʻo ka loaʻa ʻana o ka microbial DNA mai nā laʻana lepo ka hana mua i ka noiʻi lepo a ʻo ke kaʻina koʻikoʻi loa no ka holomua o nā hoʻokolohua ma hope. Eia nō naʻe, me ka hoʻohui ʻana i nā kumuwaiwai microbial waiwai, loaʻa pinepine ka lepo i ka nui o nā metabolites (humic acid, xanthic acid a me nā mea humic ʻē aʻe), hiki ke hoʻomaʻemaʻe maʻalahi ʻia me nā nucleic acid i ka wā o ke kaʻina hana unuhi nucleic acid, e hoʻopilikia ana i ka PCR ma hope a me ke kaʻina hana sequencing.Iʻa NuiHiki i ka Sequencing Soil and Faecal Genomic DNA Purification Kit ke unuhi wikiwiki a me ka maikaʻi i ka DNA genomic maʻemaʻe a me ka nui o nā laʻana waiwai i ka humus e like me nā ʻōpala lepo, he mea kōkua nui ia no ka noiʻi ʻokoʻa o ka ʻōnaehana microbial lepo.