ʻO ka lepo, ma ke ʻano he ʻano kaiaola like ʻole, he waiwai nui i nā kumuwaiwai microbial, me ka nui o nā ʻano microbial e like me ka bacteria, fungi, viruses, cyanobacteria, actinomycetes, protozoa a me nematodes. Loaʻa i ka nui o nā hana metabolic a me nā waiwai physiological, pāʻani lākou i ke kuleana koʻikoʻi i ke kaʻa kaʻa meaʻai lepo a pono no ka wehe ʻana i nā mea haumia lepo. ʻOiai ʻo ia kekahi o nā kaiapuni ʻokoʻa like ʻole o ka honua, ʻo ka noiʻi ʻana i ka biology molecular no ka lepo he mea koʻikoʻi koʻikoʻi koʻikoʻi. Ma kēia kaʻina hana, ʻo ka loaʻa ʻana o ka DNA microbial mai nā laʻana lepo ka hana mua i ka noiʻi ʻāina a me ka hana koʻikoʻi no ka holomua o nā hoʻokolohua lalo. Eia nō naʻe, ma kahi o nā kumuwaiwai microbial waiwai, loaʻa pinepine ka lepo i ka nui o nā metabolites (humic acid, xanthic acid a me nā mea humic ʻē aʻe), hiki ke hoʻomaʻemaʻe maʻalahi me nā waikawa nucleic i ka wā o ke kaʻina hana nucleic acid extraction, e pili ana i ka downstream PCR a me ke kaʻina hana.Iʻa NuiHiki i ka Sequencing Soil and Faecal Genomic DNA Purification Kit me ka hikiwawe ke unuhi i ka DNA genomic maʻemaʻe a paʻa loa mai nā laʻana waiwai humus e like me nā faeces lepo, he mea kōkua ikaika ia no ka noiʻi ʻokoʻa ʻana o ka microbial ecosystem diversity.
Huahana I'a Nui
Hoʻohana ka huahana i kahi ʻōnaehana paʻa kūʻokoʻa i hoʻomohala kūikawā a i hoʻopaʻa ʻia a me nā peʻa magnetic e hoʻopaʻa pono i ka DNA, hiki ke hoʻopaʻa koke a adsorb, hoʻokaʻawale a hoʻomaʻemaʻe i nā ʻakika nucleic, i mea kūpono no ka hoʻokaʻawale wikiwiki a maikaʻi hoʻi a me ka hoʻomaʻemaʻe ʻana i ka DNA genomic mai ka lepo a me nā faeces, a me ka wehe ʻana i nā koena e like me ka humic acids, proteins, ion paʻakai, a me nā mea ʻē aʻe. Hoʻohālikelike me ka Beaglefly sequencing magnetic bead method nucleic acid extractor, kūpono loa ia no ka unuhi ʻana o ka nui o ka laʻana. ʻO ka DNA genomic i unuhi ʻia he maʻemaʻe kiʻekiʻe a me ka maikaʻi a hiki ke hoʻohana nui ʻia ma lalo o PCR/qPCR, NGS a me nā noiʻi hoʻokolohua ʻē aʻe.
Nā hiʻohiʻona
maikaʻi maikaʻi:ka hoʻokaʻawale a me ka hoʻomaʻemaʻe ʻana i ka genomic DNA, kiʻekiʻe kiʻekiʻe, maʻemaʻe maikaʻi.
Nui nā laʻana:hiki ke hoʻohana nui ʻia i nā ʻano ʻano lepo a me nā laʻana faecal.
wikiwiki a maʻalahi:ka unuhi ʻakomi me ka mea hoʻoheheʻe like, kūpono loa no ka unuhi ʻana i nā ʻano nui.
Palekana a ʻaʻohe mea ʻawaʻawa:ʻAʻole pono i nā mea hoʻokalakupua ʻawaʻawa e like me phenol/chloroform, etc.
Nā mea kani hiki ke hoʻololi:BFEX-32/ BFEX-32E/ BFEX-96E
Ka manawa hoʻouna: Jul-10-2025