中文网站
Hiki ke hoʻokaʻawale ʻia nā ʻiʻo holoholona i nā ʻiʻo epithelial, nā ʻiʻo hoʻopili, nā ʻiʻo muscular a me nā ʻiʻo neural e like me ko lākou kumu, morphology, structure a me nā ʻano hana maʻamau, kahi i pili pū ʻia a hilinaʻi kekahi i kekahi ma nā ʻāpana like ʻole e hana i nā ʻano kino a me nā ʻōnaehana like ʻole o nā holoholona i mea e hoʻopau ai i nā ʻano hana physiological like ʻole.
ʻO ka ʻiʻo Epithelial: ua haku ʻia me nā pūnaewele epithelial i hoʻonohonoho pono ʻia a me kahi liʻiliʻi o nā pūnaewele interstitial o ke ʻano membrane-like, i uhi pinepine ʻia i loko o ka ʻili o ke kino holoholona a me ke kino o nā ʻano paipu like ʻole, nā lua, nā capsules a me ka ʻili o loko o kekahi mau ʻōpū. Loaʻa i ka ʻiʻo Epithelial nā hana o ka pale, ka huna ʻana, ka excretion a me ka omo ʻana.
ʻIʻo hoʻohui: Ua haku ʻia me nā pūnaewele a me ka nui o ka matrix intercellular. ʻO ka ʻiʻo hoʻohui i hana ʻia e ka mesoderm ka ʻano holoholona i hoʻolaha nui ʻia a ʻano like ʻole, me ka ʻiʻo hoʻohui wehe, ka ʻiʻo hoʻohui mānoanoa, ka ʻiʻo hoʻohui reticular, ka ʻiʻo cartilage, ka ʻiʻo iwi, ka ʻiʻo adipose a pēlā aku. Loaʻa iā ia nā hana o ke kākoʻo, ka hoʻohui, ka pale, ka pale ʻana, ka hoʻoponopono a me ka lawe ʻana.
ʻIʻo ʻiʻo: ua haku ʻia me nā ʻiʻo ʻiʻo i loaʻa ka hiki ke ʻaelike. ʻO ke ʻano o nā ʻiʻo ʻiʻo he lahilahi e like me ka fiber, no laila ua kapa ʻia hoʻi he fiber muscle. ʻO ka hana nui o ka fiber muscle, ʻo ia ke ʻaelike a me ka hana ʻana i ka neʻe ʻana o nā ʻiʻo. Wahi a ke ʻano a me ke ʻano o nā ʻiʻo ʻiʻo a me nā hana like ʻole, hiki ke hoʻokaʻawale ʻia nā ʻiʻo ʻiʻo i nā ʻiʻo iwi (ʻiʻo transverse), nā ʻiʻo laumania a me nā ʻiʻo puʻuwai.
ʻIʻo aʻalolo: ʻiʻo i haku ʻia me nā hunaola aʻalolo a me nā hunaola glial. ʻO nā hunaola aʻalolo nā ʻāpana morphological a me nā hana o ka ʻōnaehana aʻalolo a loaʻa iā lākou ka hiki ke ʻike i nā mea hoʻonāukiuki kūloko a me waho a alakaʻi i nā impulses i loko o ke kino.
Huahana Iʻa Nui
Hoʻohana ka huahana i kahi ʻōnaehana buffer kū hoʻokahi i hoʻomohala kūikawā ʻia a hoʻomaikaʻi ʻia a me nā momi magnetic e hoʻopaʻa pono ana i ka DNA, hiki ke hoʻopaʻa koke a hoʻopili, hoʻokaʻawale a hoʻomaʻemaʻe i nā waikawa nucleic. He kūpono ia no ka unuhi pono ʻana a me ka hoʻomaʻemaʻe ʻana i ka DNA genomic mai nā ʻano holoholona āpau a me nā ʻōpū o loko (me nā meaola kai), a hiki iā ia ke hoʻopau loa i nā ʻano protein, nā momona a me nā hui organik ʻē aʻe a me nā haumia ʻē aʻe. Hiki ke hoʻohana ʻia me kaIʻa nuiʻO ke ʻano hana Magnetic Bead Nucleic Acid Extractor, kahi mea kūpono loa no ka unuhi ʻakomi ʻana o nā nui laʻana nui. He maʻemaʻe a maikaʻi nā huahana nucleic acid i unuhi ʻia, a hiki ke hoʻohana nui ʻia ma ka PCR/qPCR ma hope, NGS, Southern hybridisation a me nā noiʻi hoʻokolohua ʻē aʻe.
Nā Hiʻohiʻona:
ʻO ka laulā o nā laʻana: hiki ke unuhi pololei ʻia ka DNA genomic mai nā ʻano laʻana holoholona āpau
Palekana a ʻaʻohe ʻawahia: ʻaʻohe mea hoʻoheheʻe ʻawahia e like me ka phenol, chloroform, a me nā mea ʻē aʻe i loko o ka reagent, me ke kumu palekana kiʻekiʻe.
ʻAna Hana: hiki ke hoʻohana ʻia ka hoʻohālikelike ʻana me ka Bigfish Nucleic Acid Extractor no ka unuhi ʻana i ka throughput kiʻekiʻe, kūpono loa no ka unuhi ʻana i ka nui o ka hāpana
Maʻemaʻe kiʻekiʻe: hiki ke hoʻohana ʻia no nā hoʻokolohua biology molekala, e like me PCR, digestion enzyme a me ka hybridisation pololei
Nā Mea Hana Pili: BFEX-32/BFEX-32E/BFEX-96E
Kaʻina hana unuhi:
Laʻana: 25-30mg o nā ʻiʻo holoholona
Wili ʻana: wili naikokene wai, wili wili a ʻokiʻoki paha
ʻO ka hoʻoheheʻe ʻana: 56 ℃ ʻauʻau mahana ʻana
Ke kau ʻana ma luna o ka moku: centrifugation e wehe i ka supernatant, a hoʻohui i ka pā hohonu no ka unuhi ʻana ma luna o ka moku.
ʻIkepili hoʻokolohua: Ua lawe ʻia he 30mg o nā hāpana ʻiʻo mai nā ʻāpana like ʻole o nā ʻiole a ua hana ʻia ka unuhi ʻana a me ka hoʻomaʻemaʻe ʻana o ka DNA me BFMP01R e like me nā kuhikuhi. Ua hōʻike nā hopena hoʻokolohua he maikaʻi ka wikiwiki o ka unuhi ʻana o ka pahu BFMP01R.
Ka manawa hoʻouna: Iulai-17-2025
Nā hoʻonohonoho pilikino