ʻO ka pahu hoʻomaʻemaʻe DNA ʻo MagPure Plasmid

Wehewehe Pōkole:

Hoʻohana kēia pahu i kahi ʻōnaehana buffer kū hoʻokahi i hoʻomohala ʻia a hoʻomaikaʻi ʻia a me nā momi magnetic e hoʻopaʻa pono i ka DNA, hiki ke hoʻopaʻa koke, hoʻopili, hoʻokaʻawale a hoʻomaʻemaʻe i nā waikawa nucleic. He kūpono loa ia no ka hoʻokaʻawale wikiwiki a me ka hoʻomaʻemaʻe ʻana i ka plasmid DNA mai ka wai bacteria 0.5-2mL (maʻamau 1-1.5mL), me ka wehe ʻana i nā koena e like me nā protein a me nā ion paʻakai. Ma ke kākoʻo ʻana i ka hoʻohana ʻana o Bigfish Magnetic Bead Nucleic Acid Extractor, he kūpono loa ia no ka unuhi ʻakomi o nā nui laʻana nui. Loaʻa i ka DNA plasmid i unuhi ʻia ka maʻemaʻe kiʻekiʻe a me ke ʻano maikaʻi, a hiki ke hoʻohana nui ʻia i nā hoʻokolohua ma hope e like me ka digestion enzyme, ligation, transformation, NGS, etc..