2× SYBR ʻōmaʻomaʻo qPCR MIX (Me ROX kiʻekiʻe)

Nā hiʻohiʻona huahana

ʻO kēia huahana, 2×SYBR ʻōmaʻomaʻo qPCR MIX, hele mai i loko o ka paipu hoʻokahi i loaʻa nā mea āpau e pono ai no ka hoʻonui ʻana a me ka ʻike ʻana o ka PCR, me Taq DNA poLymerase, SYBR green I dye, High ROX Reference Dye, dNTPs, Mg2+, a me PCR buffer.
ʻO SYBR'ōmaʻomaʻo I dye he'ōmaʻomaʻo'ōmaʻomaʻo'ōmaʻomaʻo'ōmaʻomaʻo e hoʻopaʻa i ka DNA kaulua (double-strand DNA, dsDNA) pālua helix minor groove region.SYBR green I fluoresces weakly in the free state, but once it bied to double-stranded DNA , hoʻonui nui ʻia kona fluorescence. ʻO kēia ka mea hiki ke helu i ka nui o ka DNA stranded pālua i hana ʻia ma ka PCR amplification ma ka ʻike ʻana i ka ikaika fluorescence.
Hoʻohana ʻia ʻo ROX ma ke ʻano he kala hoʻoponopono e hoʻoponopono ai i ka fluorescence fluctuations pili ʻole i ka PCR, no laila e hōʻemi ana i nā ʻokoʻa spatial. Hiki ke kumu o ia mau ʻokoʻa ma muli o nā kumu like ʻole, e like me ka hewa pipette a i ʻole ka hoʻoheheʻe ʻana. Loaʻa nā pono like ʻole o nā mea hoʻohālikelike fluorescence no ROX, a ua kūpono kēia huahana no nā ananaly quantification fluorescence e koi ana i ka hoʻoponopono kiʻekiʻe ROX.