2× SYBR ʻōmaʻomaʻo qPCR MIX（Me ke kiʻekiʻe ROX）

Nā hiʻohiʻona huahana

ʻO kēia huahana, ʻo 2×SYBR green qPCR MIX, hele mai i loko o kahi ʻōmole hoʻokahi e loaʻa ana nā ʻāpana āpau e pono ai no ka hoʻonui ʻana a me ka ʻike ʻana i ka PCR, me Taq DNA poLymerase, SYBR green I dye, High ROX Reference Dye, dNTPs, Mg2+, a me ka PCR buffer.
ʻO ka dye ʻōmaʻomaʻo I SYBR kahi dye fluorescent ʻōmaʻomaʻo e hoʻopaʻa i ka DNA kaula pālua (DNA kaula pālua, dsDNA) ʻāpana groove helix pālua. Hoʻomālamalama nāwaliwali ʻo SYBR ʻōmaʻomaʻo I i ke kūlana manuahi, akā i ka wā e hoʻopaʻa ai i ka DNA kaula pālua, hoʻonui nui ʻia kona fluorescence. ʻO kēia ka mea e hiki ai ke helu i ka nui o ka DNA kaula pālua i hana ʻia i ka wā o ka hoʻonui ʻana o PCR ma ka ʻike ʻana i ka ikaika fluorescence.
Hoʻohana ʻia ʻo ROX ma ke ʻano he kala hoʻoponopono e hoʻoponopono ai i nā loli fluorescence i pili ʻole i ka PCR, no laila e hōʻemi ana i nā ʻokoʻa spatial. Hiki ke hana ʻia kēlā mau ʻokoʻa e nā kumu like ʻole, e like me ka hewa pipette a i ʻole ka mahu ʻana o ka laʻana. Loaʻa i nā mea hana helu fluorescence like ʻole nā ​​​​pono like ʻole no ROX, a kūpono kēia huahana no nā mea kālailai helu fluorescence e pono ai ka hoʻoponopono ROX kiʻekiʻe.