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Ka holomua pili
Wahi a ke Keʻena ʻIke o ke Kuhina o ka mahiʻai a me nā wahi kua'āina, i ʻAukake 2018, ua loaʻa kahi maʻi puaʻa ʻApelika ma Shenbei New District, Shenyang City, Liaoning Province, ʻo ia ka maʻi puaʻa ʻApelika mua ma Kina. Ma Ianuali 14, 2019, ua loaʻa ka maʻi puaʻa ʻApelika ma mua o 20 mau panalāʻau ma Kina, e pepehi ana i nā puaʻa 916000, e hopohopo nei i ka lehulehu.
ʻO ka maʻi puaʻa ʻApelika (ASF)
ʻO ka ASF (African Swine Fever) he maʻi lele nui ia i hoʻokumu ʻia e ka maʻi maʻi puaʻa puaʻa ʻApelika (ACFV) e hoʻopili ana i nā puaʻa hale a me nā puaʻa ʻāhiu (African wild boar, European wild boar, etc.). Ua helu ʻia ka hui honua no ke olakino holoholona (OIE) ma ke ʻano he maʻi holoholona i hōʻike ʻia e ke kānāwai, ʻo ia hoʻi ke ʻano o ka maʻi maʻi holoholona a Kina e nānā ai i ka pale ʻana.
Nā ʻano o ka maʻi
ʻO nā hōʻike maʻi ke kuni (a hiki i 40 ~ 42 ℃), tachycardia, dyspnea, ʻāpana ʻāʻī, serous a mucinous purulent secretion i nā maka a me ka ihu, cyanosis ʻili, ʻike ʻia ke koko o ka puʻupaʻa, lymph node a me ka mucosa gastrointestinal. ʻO nā hōʻailona lapaʻau o ka maʻi maʻi puaʻa maʻamau o ʻApelika e like me ka maʻi puaʻa maʻamau, hiki ke ʻike wale ʻia e ka nānā ʻana i ka laboratory.
ʻO ka hopena i ka maʻi puaʻa maʻamau o ʻApelika
1. Ka hana laʻana
He kūpono ia no ke koko a me nā ʻiʻo like ʻole o nā puaʻa maʻi i manaʻo ʻia: spleen, lymph node a me nā ʻiʻo puʻupaʻa.
Nā laʻana koko
E lawe i 200 μ L koko hāpana, 5000g centrifugal 5 min, e lawe i ka supernatant no ka nānā ʻana.
Nā laʻana kiko
Ma hope o ka hoʻomaloʻo ʻia ʻana o nā mea kiko, ua hoʻohui ʻia ka nui kūpono o ka saline maʻamau a i ʻole PBS, a ua centrifuged ka supernatant e hoʻāʻo ʻia.
2. Wehe 'akomi
ʻO Hangzhou Bigfish Bio-tech Co., Ltd. Hiki i ka BFEX-32 ka mea hoʻopihapiha nucleic acid ke hoʻopau i ka unuhi ʻana o 32 mau hiʻohiʻona i 30 mau minuke, hiki ke hoʻokō i kāu mau koi no ka unuhi ʻana i kahi helu nui o nā hōʻailona. ʻAʻole pono ka hana holoʻokoʻa i kahi hana ʻē aʻe, mālama i ka manawa a hōʻemi i ka hewa o ka hana lima, a hoʻomaikaʻi i ka hana pono e like me ka hiki.
3. ʻO ka hoʻomaʻemaʻe hoʻomaʻemaʻe nucleic acid kiʻekiʻe
ʻO MagPure Nucleic acid extraction kit, me BFEX-32, ua kūlike ka huahana i ka ʻike PCR & QPCR.
4. Ka ho'āʻo kamepiula a me ka nānā ʻana
E like me ke kūlana maoli o ka hale hana, hoʻohana ka mea hoʻohana i ka qualitative a quantitative detection scheme.
Hāʻawi ʻo Hangzhou Bigfish Bio-tech Co., Ltd. i kahi pahu ʻike qualitative (Quantitative) no ka maʻi puaʻa maʻamau o ʻApelika (ACFV) me FC-96G (BFQP-16/48), hiki ke ʻike i ka ACFV nui, maʻalahi a hilinaʻi.
ʻO nā mea hou aʻe, e ʻoluʻolu e hoʻolohe i ka moʻolelo kūhelu WeChat o Hangzhou Bigfish Bio-tech Co., Ltd.
Ka manawa hoʻouna: Mei-23-2021
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