Pahu Hoʻomaʻemaʻe DNA Swab Waha Bigfish FeiXu: He Hoʻonā Kūpono Loa no ka Wehe ʻana i ka DNA Laʻana Waha

Ma nā kahua onā hōʻailona lapaʻau in vitro (IVD), genotyping, a me ka noiʻi molekala, nā hāpana waha—e like menā ʻūpā waha, nā ʻūpā puʻu, a me ka lawai—hoʻohana nui ʻia no ka hoʻāʻo ʻana i ka waikawa nucleic ma muli o ko lākouka hōʻiliʻili maʻalahi, ke ʻano invasive ʻole, a me ke kaʻina hana laʻana ʻeha ʻoleEia nō naʻe, loaʻa pinepine nā hāpana wahanā palena palena o nā waikawa nucleica ua haumia pinepine ʻia menā protein a me nā mea haumia ʻē aʻeHoʻopilikia pinepine ʻia nā ʻano unuhi kuʻunanā kaʻina hana paʻakikī, ka pono haʻahaʻa, a me ka hoʻohana ʻana i nā reagents ʻawahia, hiki ke hoʻopilikia nui i ka pololei a me ke kūpaʻa o nā noi ma hope e like mePCR/qPCR a me ka hoʻonohonoho ʻana o ka hanauna hou (NGS).

ʻO kaPahu Hoʻokaʻawale DNA Genomic me ka Swab Waha Magnetic BFMP06, i hoʻomohala ʻia eʻO ka ʻenehana ʻenehana ʻo Hangzhou Bigfish FeiXu, hāʻawi i kahihopena palekana, kūpono, a hilinaʻi hoʻino ka unuhi ʻana i ka DNA hāpana waha. Me kāna hoʻolālā loea hou a me nā kūlana hana koʻikoʻi, ua lilo kēia pahu i mea hana hilinaʻi no nā keʻena hoʻokolohua lapaʻau a me nā noiʻi.

Ua kūkulu ʻia ka pahu BFMP06 a puni kahiʻōnaehana buffer i hoʻonohonoho pono ʻiai hui pū ʻia meNā momi magnetic hydroxyl kikoʻī DNA, e hana ana i kahi kahe hana hoʻomaʻemaʻe waikawa nucleic kūpono loa. Ma hope o ka lysed ʻana o ka laʻana i loko o ka lysis buffer, ua hoʻopilikia ʻia nā ʻāpana cellular a hoʻokuʻu ʻia nā waikawa nucleic. Hoʻopaʻa koho nā hui hana ma ka ʻili o nā momi magnetic i ka DNA manuahi, e hana ana i ka paʻanā hui pū ʻana o ka bead-DNA magnetic.

Ma lalo o kahi kahua magnetic waho, ke hele nei nā complexesʻelua mau ʻanuʻu holoi pololeie hoʻopau pono i nā protein, nā paʻakai, a me nā mea haumia ʻē aʻe. ʻO ka hope loa,DNA genomic maʻemaʻe kiʻekiʻeua eluted maikaʻi ʻia me ka hoʻohana ʻana i ka elution buffer.

Wehewehe Huahana