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ʻO ka laiki kekahi o nā mea kanu nui loa, no nā mea kanu wai o ka ʻohana Poaceae. ʻO Kina kekahi o nā wahi noho mua o ka laiki, i mahi nui ʻia ma ka ʻaoʻao hema o Kina a me ka ʻāina ʻĀkau Hikina. Me ka holomua o ka ʻepekema a me ka ʻenehana, hoʻohana nui ʻia nā ʻenehana biology molecular hou i ka noiʻi laiki. ʻO ka loaʻa ʻana o ka DNA genomic raiki kiʻekiʻe a me ka maʻemaʻe kiʻekiʻe e waiho i kahi kumu paʻa no nā haʻawina genetic downstream. ʻO ka BigFish Sequence Magnetic Bead-Based Rice Genomic DNA Purification Kit e hiki ai i nā mea noiʻi raiki ke unuhi i ka DNA raiki maʻalahi, wikiwiki, a me ka maikaʻi.
Puke Hoʻomaʻemaʻe DNA Raiki Genome
Nānā Huahana:
Hoʻohana kēia huahana i kahi ʻōnaehana pale kūʻokoʻa i kūkulu ʻia a hoʻopaʻa pono ʻia a me nā peʻa magnetic me nā waiwai paʻa DNA kikoʻī. Hoʻopaʻa wikiwiki ia, hoʻopili, a hoʻokaʻawale i nā ʻakika nucleic oiai e wehe pono ana i nā mea haumia e like me polysaccharides a me nā pūhui polyphenolic mai nā mea kanu. He kūpono loa ia no ka unuhi ʻana i ka DNA genomic mai nā ʻiʻo lau mea kanu. Hoʻohui pū ʻia me ka BigFish Magnetic Bead Nucleic Acid Extraction Instrument, kūpono ia no ka ʻohi ʻana i nā puʻupuʻu nui. Hōʻike nā huahana nucleic acid i ka maʻemaʻe kiʻekiʻe a me ka maikaʻi maikaʻi loa, e hoʻohana nui ʻia no ka noiʻi hoʻokolohua o lalo e like me PCR/qPCR a me NGS.
Nā hiʻohiʻona huahana:
Palekana a ʻaʻohe mea ʻawaʻawa: ʻAʻohe pono o nā mea hoʻokalakupua kūlohelohe e like me phenol/chloroform
Hoʻopili kiʻekiʻe kiʻekiʻe: Hoʻopili ʻia me ka Beagle sequencing nucleic acid extractor, hiki iā ia ke hana i ka hoʻoiho kiʻekiʻe-throughput a kūpono no ka unuhi ʻana i nā ʻano nui.
ʻO ka maʻemaʻe kiʻekiʻe a me ka maikaʻi maikaʻi: ʻO ka huahana i unuhi ʻia he maʻemaʻe kiʻekiʻe a hiki ke hoʻohana ʻia no ka NGS lalo, ka hoʻohuihui chip a me nā hoʻokolohua ʻē aʻe.
Nā mea kani kūpono: BigFish BFEX-32/BFEX-32E/BFEX-96E
Ka manawa hoʻouna: Sep-11-2025
Nā hoʻonohonoho pilikino