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ʻO ka laiki kekahi o nā mea kanu koʻikoʻi loa, no nā mea kanu wai o ka ʻohana Poaceae. ʻO Kina kekahi o nā wahi noho mua o ka laiki, i mahi nui ʻia ma ka hema o Kina a me ka ʻāina ʻĀkau Hikina. Me ka holomua o ka ʻepekema a me ka ʻenehana, ua hoʻohana nui ʻia nā ʻano hana biology molecular hou i ka noiʻi laiki. ʻO ka loaʻa ʻana o ka DNA genomic laiki kiʻekiʻe a maʻemaʻe hoʻi e kau ana i kahi kahua paʻa no nā haʻawina genetic ma hope. ʻO ka BigFish Sequence Magnetic Bead-Based Rice Genomic DNA Purification Kit e hiki ai i nā mea noiʻi laiki ke unuhi i ka DNA laiki me ka maʻalahi, wikiwiki, a me ka pono.
Pahu Hoʻomaʻemaʻe DNA Genome Laiki
ʻIke Huahana:
Hoʻohana kēia huahana i kahi ʻōnaehana buffer kū hoʻokahi i hoʻomohala kūikawā ʻia a hoʻomaikaʻi ʻia a me nā momi magnetic me nā waiwai hoʻopaʻa DNA kikoʻī. Hoʻopaʻa koke ia, hoʻopili, a hoʻokaʻawale i nā waikawa nucleic me ka wehe pono ʻana i nā haumia e like me nā polysaccharides a me nā hui polyphenolic mai nā mea kanu. He kūpono loa ia no ka unuhi ʻana i ka DNA genomic mai nā ʻiʻo lau mea kanu. Hoʻohui ʻia me ka BigFish Magnetic Bead Nucleic Acid Extraction Instrument, he kūpono ia no ka unuhi ʻakomi ʻana o nā nui laʻana nui. Hōʻike nā huahana waikawa nucleic i unuhi ʻia i ka maʻemaʻe kiʻekiʻe a me ke ʻano maikaʻi loa, e hoʻohana nui ʻia no ka noiʻi hoʻokolohua ma hope e like me PCR/qPCR a me NGS.
Nā Hiʻohiʻona Huahana:
Palekana a ʻaʻohe ʻawahia: ʻAʻohe pono no nā reagents organik ʻawahia e like me ka phenol/chloroform
Ka hoʻopuka kiʻekiʻe aunoa: Hoʻohui ʻia me kahi mea hoʻokaʻawale waikawa nucleic sequencing Beagle, hiki iā ia ke hana i ka unuhi ʻana i ka throughput kiʻekiʻe a kūpono no ka unuhi ʻana i nā nui laʻana nui
Maʻemaʻe kiʻekiʻe a me ka maikaʻi maikaʻi: ʻO ka huahana i unuhi ʻia he maʻemaʻe kiʻekiʻe a hiki ke hoʻohana ʻia no ka NGS ma hope, ka hybridization chip a me nā hoʻokolohua ʻē aʻe.
Nā mea hana kūpono: BigFish BFEX-32/BFEX-32E/BFEX-96E
Ka manawa hoʻouna: Sep-11-2025
Nā hoʻonohonoho pilikino